6IEA

Structure of RVFV Gn and human monoclonal antibody R13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	42.210, 97.107, 112.504
Unit cell angles	90.00, 96.30, 90.00

Refinement procedure

Resolution	48.454 - 2.000
R-factor	0.1829
Rwork	0.181
R-free	0.22540
RMSD bond length	0.008
RMSD bond angle	1.005
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rpim	0.041	0.365
Number of reflections	61226
<I/σ(I)>	16.4	2
Completeness [%]	99.9	100
Redundancy	6.8	6.9
CC(1/2)	0.992	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M magnesium formate, 20% w/v PEG 3350