6IE8
RamR in complex with cholic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.207, 53.613, 43.941 |
| Unit cell angles | 90.00, 93.22, 90.00 |
Refinement procedure
| Resolution | 43.870 - 2.000 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.23210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vvx |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.998 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (10.2.35) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.068 | 0.033 | 0.521 |
| Total number of observations | 104226 | ||
| Number of reflections | 13733 | 682 | |
| <I/σ(I)> | 11 | ||
| Completeness [%] | 99.1 | 94.2 | 99.1 |
| Redundancy | 7.6 | 6.8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M MES pH6.5, 0.2M Ammonium Sulfate, 20% PEG6000 |
