6IE1
Crystal Structure of ELMO2(Engulfment and cell motility protein 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-20 |
Detector | DECTRIS PILATUS3 X 1M |
Wavelength(s) | 0.97853 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 75.182, 119.134, 169.326 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.331 - 2.480 |
R-factor | 0.2074 |
Rwork | 0.206 |
R-free | 0.23970 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.733 |
Data reduction software | d*TREK |
Data scaling software | HKL-3000 |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.520 |
High resolution limit [Å] | 2.480 | 6.730 | 2.480 |
Rmerge | 0.068 | 0.030 | 0.920 |
Rmeas | 0.071 | 0.032 | 0.960 |
Rpim | 0.020 | 0.009 | 0.269 |
Number of reflections | 27341 | 1486 | 1366 |
<I/σ(I)> | 4.9 | ||
Completeness [%] | 99.9 | 99.9 | 99.8 |
Redundancy | 12.6 | 12.9 | 12.4 |
CC(1/2) | 0.999 | 0.898 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M Sodium malonate pH 4.0, 12%PEG3,350 |