Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 310.369, 86.594, 82.314 |
| Unit cell angles | 90.00, 93.67, 90.00 |
Refinement procedure
| Resolution | 154.870 - 2.780 |
| R-factor | 0.2212 |
| Rwork | 0.220 |
| R-free | 0.25310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jx5 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 154.870 | 10.000 | 2.900 |
| High resolution limit [Å] | 2.780 | 6.020 | 2.800 |
| Rmerge | 0.055 | 0.033 | 0.247 |
| Number of reflections | 52523 | 5282 | 5087 |
| <I/σ(I)> | 29.6 | ||
| Completeness [%] | 97.2 | 95.3 | 94.6 |
| Redundancy | 4.3 | 4.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10% PEG 6K, 0.1M ammonium phosphate buffer, 2% MPD |
