6IDP
Crystal structure of Vibrio cholerae MATE transporter VcmN in the straight form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-15 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.225, 92.027, 101.302 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.014 - 2.205 |
R-factor | 0.2008 |
Rwork | 0.198 |
R-free | 0.24050 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.782 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.014 | 46.014 | 2.340 |
High resolution limit [Å] | 2.210 | 6.560 | 2.210 |
Rmerge | 0.155 | 0.035 | 1.269 |
Rmeas | 0.167 | 0.038 | 1.397 |
Total number of observations | 209892 | ||
Number of reflections | 29962 | 1275 | 4655 |
<I/σ(I)> | 10.51 | 37 | 1.37 |
Completeness [%] | 99.6 | 99.5 | 97.9 |
Redundancy | 7.005 | 6.578 | 5.69 |
CC(1/2) | 0.997 | 0.999 | 0.539 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | PEG500DME, Tris-HCl, magnesium formate |