6IDN
Crystal structure of ICChI chitinase from ipomoea carnea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.98401 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.490, 59.490, 171.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hvq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.896 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.068 | 0.280 |
| Number of reflections | 50243 | 50372 |
| <I/σ(I)> | 20.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 5mM Cobalt Chloride, 5mM Cadmium Chloride, 5mM Magnesium Chloride, 5mM Nickle Chloride, 100mM HEPES pH 7.0, 11% PEG 3350 |
