6IDB
Crystal structure of H7 hemagglutinin mutant H7-SVTQ ( A138S, P221T, L226Q) with 6'SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.03907 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 115.956, 115.956, 297.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.510 - 2.502 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kol |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.069 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.032 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.146 | 1.081 |
| Number of reflections | 26969 | 2320 |
| <I/σ(I)> | 18.79 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 25% w/v SOKALAN PA 25 CL |
