6ID8
Crystal structure of H7 hemagglutinin mutant H7-SVTL ( A138S, P221T) from the influenza virus A/Anhui/1/2013 (H7N9)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97892 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 117.243, 117.243, 294.129 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.434 - 2.902 |
| R-factor | 0.2436 |
| Rwork | 0.242 |
| R-free | 0.27120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kol |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.570 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 16810 | |
| <I/σ(I)> | 21.42 | |
| Completeness [%] | 95.5 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 2.0M Ammonium sulfate, 0.1M Sodium HEPES pH 7.5, 5% v/v PEG 400 |
