6ID5

Crystal structure of H7 hemagglutinin mutant H7-SVPL ( A138S) from the influenza virus A/Anhui/1/2013 (H7N9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.91847
Spacegroup name	H 3 2
Unit cell lengths	115.386, 115.386, 294.486
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.585 - 2.600
R-factor	0.2612
Rwork	0.259
R-free	0.31030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kol
RMSD bond length	0.004
RMSD bond angle	0.716
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	23529
<I/σ(I)>	20.16
Completeness [%]	99.6
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.8M Ammonium sulfate, 0.1M BIS-TRIS pH 6.5, 2% v/v Polyethylene glycol monomethyl ether 550