6ID3

Crystal structure of H7 hemagglutinin mutant H7-SGPL ( A138S, V186G) from the influenza virus A/Anhui/1/2013 (H7N9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97923
Spacegroup name	H 3 2
Unit cell lengths	115.629, 115.629, 294.096
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.384 - 2.600
R-factor	0.2492
Rwork	0.248
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kol
RMSD bond length	0.002
RMSD bond angle	0.866
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	23387
<I/σ(I)>	11.68
Completeness [%]	98.6
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2.0M Ammonium sulfate, 0.1M Sodium HEPES pH 7.5, 5% v/v PEG 400