6ICW
Crystal structure of H7 hemagglutinin mutant AH-SGTQ (A138S, V186G, P221T and L226Q) from the influenza virus A/Anhui/1/2013 (H7N9)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 115.605, 115.605, 293.498 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.530 - 2.600 |
| R-factor | 0.2588 |
| Rwork | 0.258 |
| R-free | 0.28350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lkg |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.930 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.530 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 23594 | |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Sodium citrate tribasic dihydrate, 20% w/v Polyethylene glycol 3350 |
