6ICL
Pseudomonas putida CBB5 NdmB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 98.553, 98.553, 174.441 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.452 - 2.100 |
R-factor | 0.1973 |
Rwork | 0.196 |
R-free | 0.22170 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.137 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.168 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmeas | 0.062 | |
Number of reflections | 30303 | |
<I/σ(I)> | 60 | |
Completeness [%] | 94.6 | |
Redundancy | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris-HCl pH 8.5, 0.5M NaCl, 5.36% w/v PEG 8000, 20.1% v/v MPD |