6ICF
The NZ-1 Fab complexed with the PDZ tandem fragment of A. aeolicus S2P homolog with the PA12 tag inserted between the residues 263 and 266
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-19 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1000 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 171.860, 171.860, 118.016 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.640 | 4.470 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.186 | 0.744 |
Number of reflections | 7742 | 2144 |
<I/σ(I)> | 12.2 | 3.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 13.5 | 12.5 |
CC(1/2) | 0.996 | 0.911 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% (wt./vol.) PEG 3350 200 mM ammonium citrate (pH 7.0) |