6ICD

REGULATION OF AN ENZYME BY PHOSPHORYLATION AT THE ACTIVE SITE

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	105.100, 105.100, 150.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.800
R-factor	0.163 *
Rwork	0.163
RMSD bond length	0.016 *
RMSD bond angle	3.300
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	NaN *
Total number of observations	89070 *
Number of reflections	20711 *
Completeness [%]	79.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.4 *		took J. H. Hurley et al.,from original paper *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	28 (mg/ml)
2	1	1	ammonium sulfate	34 (%sat)
3	1	1		100 (mM)
4	1	1		35 (mM)
5	1	1	citric acid	9 (mM)
6	1	1	dithiothreitol	0.2 (mM)