6ICC
The NZ-1 Fab complexed with the PDZ tandem fragment of A. aeolicus S2P homolog with the PA12 tag inserted between the residues 181 and 186
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9800 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.938, 84.293, 185.913 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.230 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.056 | 0.874 |
Number of reflections | 54069 | 3874 |
<I/σ(I)> | 12.6 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.7 | 6.7 |
CC(1/2) | 0.940 | 0.873 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12-15%(wt./vol.) PEG 3350, 200mM sodium citrate |