6IB8

Structure of a complex of SuhB and NusA AR2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-06
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	64.270, 95.540, 104.542
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.634 - 1.646
R-factor	0.1825
Rwork	0.181
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qfl 1wcn
RMSD bond length	0.015
RMSD bond angle	1.009
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.740
High resolution limit [Å]	1.646	1.646
Rmeas		1.860
Number of reflections	78162	12069
<I/σ(I)>	15.2	0.92
Completeness [%]	99.1	95.7
Redundancy	5.5	5.1
CC(1/2)	0.990	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1M Tris-HCl pH 8.5, 5% (w/v) PEG 8000, 16% (v/v) PEG 300, 10% (v/v) glycerol