6IB8
Structure of a complex of SuhB and NusA AR2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-06 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.91841 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.270, 95.540, 104.542 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.634 - 1.646 |
R-factor | 0.1825 |
Rwork | 0.181 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qfl 1wcn |
RMSD bond length | 0.015 |
RMSD bond angle | 1.009 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.740 |
High resolution limit [Å] | 1.646 | 1.646 |
Rmeas | 1.860 | |
Number of reflections | 78162 | 12069 |
<I/σ(I)> | 15.2 | 0.92 |
Completeness [%] | 99.1 | 95.7 |
Redundancy | 5.5 | 5.1 |
CC(1/2) | 0.990 | 0.360 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1M Tris-HCl pH 8.5, 5% (w/v) PEG 8000, 16% (v/v) PEG 300, 10% (v/v) glycerol |