6IAZ
The archaeal Methanocaldococcus infernus Elp3 with N-terminus deletion (1-46)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 63.350, 63.350, 250.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.818 - 1.901 |
R-factor | 0.1958 |
Rwork | 0.195 |
R-free | 0.21740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l7l |
RMSD bond length | 0.007 |
RMSD bond angle | 0.825 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmeas | 0.068 | 0.997 |
Number of reflections | 41281 | |
<I/σ(I)> | 19.34 | |
Completeness [%] | 99.8 | |
Redundancy | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M citrate (pH 3.8), 0.1 M LiCl and 17% PEG6000 |