6IAS
structure of human NKp46 in complex with antibody NKp46-1 and NKp46-4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-30 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9801 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.657, 61.101, 84.138 |
Unit cell angles | 90.00, 105.03, 90.00 |
Refinement procedure
Resolution | 36.760 - 1.750 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.250 | 1.820 |
High resolution limit [Å] | 1.725 | 1.725 |
Number of reflections | 50086 | |
<I/σ(I)> | 8.8 | |
Completeness [%] | 99.5 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MMT pH8.5-9.5, 20-30%(w/v) PEG 1500 |