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6IAS

structure of human NKp46 in complex with antibody NKp46-1 and NKp46-4

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSOLEIL BEAMLINE PROXIMA 2
Synchrotron siteSOLEIL
BeamlinePROXIMA 2
Temperature [K]100
Detector technologyPIXEL
Collection date2017-03-30
DetectorDECTRIS EIGER X 9M
Wavelength(s)0.9801
Spacegroup nameP 1 21 1
Unit cell lengths48.657, 61.101, 84.138
Unit cell angles90.00, 105.03, 90.00
Refinement procedure
Resolution36.760 - 1.750
R-factor0.197
Rwork0.195
R-free0.22800
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.009
RMSD bond angle1.050
Data reduction softwareXDS
Data scaling softwareSCALA
Phasing softwareMOLREP
Refinement softwareBUSTER (2.10.3)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]37.2501.820
High resolution limit [Å]1.7251.725
Number of reflections50086
<I/σ(I)>8.8
Completeness [%]99.5
Redundancy6.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP2930.1M MMT pH8.5-9.5, 20-30%(w/v) PEG 1500

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