6IAI
StoD is a novel Salmonella Typhi type III secretion system E3 ubiquitin ligase effector
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2013-01-23 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.972 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 92.960, 92.960, 156.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.650 - 2.540 |
R-factor | 0.2173 |
Rwork | 0.216 |
R-free | 0.24450 |
Structure solution method | SIRAS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.150 |
Data reduction software | xia2 |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.650 | 2.610 |
High resolution limit [Å] | 2.540 | 2.540 |
Number of reflections | 23299 | |
<I/σ(I)> | 30.8 | |
Completeness [%] | 99.8 | |
Redundancy | 26.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 60% 0.9 M Na malonate, 0.5% Jeffamine, 0.1M HEPES pH 6.5 |