6IAF
Fifteen minutes iron loaded Rana Catesbeiana H' ferritin variant H54N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.95372 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 183.720, 183.720, 183.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.440 - 1.350 |
R-factor | 0.11465 |
Rwork | 0.113 |
R-free | 0.14072 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lqh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.481 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 22.440 | 2.470 | 1.420 |
High resolution limit [Å] | 1.350 | 2.340 | 1.350 |
Rmerge | 0.073 | 0.780 | 0.447 |
Rmeas | 0.078 | 0.822 | 0.480 |
Rpim | 0.021 | 0.255 | 0.128 |
Number of reflections | 58512 | 1656 | 8388 |
<I/σ(I)> | 22.3 | 3.5 | 6 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 14.2 | 10.2 | 13.8 |
CC(1/2) | 0.795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 281 | 1.6-2.0 M MgCl2 and 0.1 M bicine pH 9.0 |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 281 | 1.6-2.0 M MgCl2 and 0.1 M bicine pH 9.0 |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 281 | 1.6-2.0 M MgCl2 and 0.1 M bicine pH 9.0 |