6IAD

Apo crystal structure of archaeal Methanocaldococcus infernus Elp3 (del1-54)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 41 21 2
Unit cell lengths	62.970, 62.970, 248.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.427 - 2.050
R-factor	0.198
Rwork	0.197
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l7l
RMSD bond length	0.003
RMSD bond angle	0.665
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.050	2.050
Rmeas	0.105	1.629
Number of reflections	32466
<I/σ(I)>	16.08
Completeness [%]	99.8
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M phosphate/citrate (pH 3.8), 0.2 M LiSO4 and 12% PEG1000