6IAB
Tail fiber of Staphylococcus aureus phage P68
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-05-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | H 3 |
| Unit cell lengths | 100.733, 100.733, 125.462 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.968 - 2.000 |
| R-factor | 0.1663 |
| Rwork | 0.164 |
| R-free | 0.20190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5efv |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.574 |
| Data reduction software | XDS (0.73) |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (dev_3042) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.968 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 32065 | 2146 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.16 | 200 mM magnesium chloride, 100 mM HEPES, 20% (w/v) polyethylene glycol 6000 |
