6IA8

Apo crystal structure of archaeal Methanocaldococcus infernus Elp3 (del1-19)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 41 21 2
Unit cell lengths	63.440, 63.440, 250.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.859 - 1.900
R-factor	0.1816
Rwork	0.180
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l7l
RMSD bond length	0.009
RMSD bond angle	0.840
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.900	1.900
Rmeas	0.068	1.582
Number of reflections	41022
<I/σ(I)>	33.38
Completeness [%]	98.3
Redundancy	24.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M phosphate/citrate (pH 3.8), 0.2 M LiSO4 and 12% PEG1000