6IA8
Apo crystal structure of archaeal Methanocaldococcus infernus Elp3 (del1-19)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 63.440, 63.440, 250.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.859 - 1.900 |
R-factor | 0.1816 |
Rwork | 0.180 |
R-free | 0.21470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l7l |
RMSD bond length | 0.009 |
RMSD bond angle | 0.840 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmeas | 0.068 | 1.582 |
Number of reflections | 41022 | |
<I/σ(I)> | 33.38 | |
Completeness [%] | 98.3 | |
Redundancy | 24.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M phosphate/citrate (pH 3.8), 0.2 M LiSO4 and 12% PEG1000 |