6IA7
T. brucei IFT22 GTP-bound crystal structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 61 |
Unit cell lengths | 55.845, 55.845, 263.443 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.363 - 2.300 |
R-factor | 0.2167 |
Rwork | 0.212 |
R-free | 0.26390 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 0.899 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.102 | 2.088 |
Number of reflections | 20689 | 1996 |
<I/σ(I)> | 16.7 | 0.9 |
Completeness [%] | 99.7 | 96.7 |
Redundancy | 9.9 | 8.8 |
CC(1/2) | 0.999 | 0.497 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | 20% (w/v) PEG3350, 50 mM NaCacodylate pH 6.5 and 200 mM calcium acetate |