6IA3

urate oxidase under 220 bar (22 MPa) of argon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.77
Spacegroup name	I 2 2 2
Unit cell lengths	79.787, 95.149, 104.276
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.620 - 1.690
R-factor	0.1494
Rwork	0.148
R-free	0.18240
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2iba
RMSD bond length	0.008
RMSD bond angle	1.392
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	FFT
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	1.780
High resolution limit [Å]	1.690	1.690
Rmerge	0.060	0.172
Rpim	0.016	0.116
Number of reflections	39125	2310
<I/σ(I)>	31.3
Completeness [%]	87.8	36.3
Redundancy	12.5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.5	291	20 microliter of protein (15 mg/ml) mixed with 20 microliter of solution: buffer (Tris 0.05M /Phosphate 0.05M) + 4% PEG 4000.