6I9Z
urate oxidase under 65 bar of argon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2005-07-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9775 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.323, 96.121, 105.412 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.320 - 1.600 |
| R-factor | 0.15702 |
| Rwork | 0.156 |
| R-free | 0.18296 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1r51 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.917 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | FFT |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.053 | 0.500 |
| Number of reflections | 52954 | 5301 |
| <I/σ(I)> | 19.3 | 2.2 |
| Completeness [%] | 98.0 | 99.5 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 8 | 291 | 20 microliter of protein (15 mg/ml) mixed with 20 microliter of solution: Tris 0.05M (chloride free) + 4% PEG 4000. |
