6I9T
Two minutes iron loaded Rana Catesbeiana H' ferritin variant H54N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.95372 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 183.830, 183.830, 183.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.300 - 1.200 |
R-factor | 0.12158 |
Rwork | 0.121 |
R-free | 0.13706 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lqh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.519 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 22.460 | 1.950 | 1.260 |
High resolution limit [Å] | 1.200 | 1.850 | 1.200 |
Rmerge | 0.058 | 0.737 | 0.366 |
Rmeas | 0.063 | 0.854 | 0.452 |
Rpim | 0.018 | 0.347 | 0.188 |
Number of reflections | 80466 | 3165 | 11066 |
<I/σ(I)> | 19.2 | 2.1 | 4 |
Completeness [%] | 97.2 | 95.5 | 92.9 |
Redundancy | 10.8 | 5.6 | 5.6 |
CC(1/2) | 0.999 | 0.653 | 0.860 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 281 | 1.6-2.0 M MgCl2 and 0.1 M bicine pH 9.0 |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 281 | 1.6-2.0 M MgCl2 and 0.1 M bicine pH 9.0 |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 281 | 1.6-2.0 M MgCl2 and 0.1 M bicine pH 9.0 |