6I9S
hRobo2 Extracellular Domains 2-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.220, 70.520, 97.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.660 - 2.480 |
| R-factor | 0.22925 |
| Rwork | 0.226 |
| R-free | 0.29292 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.443 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.660 | 2.580 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Number of reflections | 15692 | 1690 |
| <I/σ(I)> | 13.5 | 1.9 |
| Completeness [%] | 99.2 | 97.4 |
| Redundancy | 4.6 | |
| CC(1/2) | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M Sodium format pH 7 and 20% PEG 3350 |
