6I9Q
Structure of the mouse CD98 heavy chain ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-22 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9184 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 97.384, 97.384, 218.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.450 - 2.100 |
R-factor | 0.1771 |
Rwork | 0.175 |
R-free | 0.21840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dh2 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.583 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.450 | 34.450 | 2.200 |
High resolution limit [Å] | 2.100 | 10.000 | 2.100 |
Rmerge | 0.130 | 0.036 | 1.453 |
Rmeas | 0.133 | 0.037 | 1.480 |
Number of reflections | 31196 | 343 | 3984 |
<I/σ(I)> | 21.43 | 69.04 | 2.82 |
Completeness [%] | 99.9 | 96.1 | 99.9 |
Redundancy | 26.556 | 22.548 | 28.081 |
CC(1/2) | 1.000 | 1.000 | 0.811 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 24 % (w/v) PEG 3350, 0.1 M Bis-tris propane/HCl, 0.2 M sodium sulfate |