6I9P
Iron-free state of Rana catesbeiana H' ferritin variant H54N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8856 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 183.910, 183.910, 183.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.100 - 1.250 |
| R-factor | 0.11771 |
| Rwork | 0.117 |
| R-free | 0.13327 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lqh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.477 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.240 | 1.320 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.074 | 0.418 |
| Rmeas | 0.079 | 0.450 |
| Rpim | 0.021 | 0.122 |
| Number of reflections | 73567 | 10565 |
| <I/σ(I)> | 19.1 | 6.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.1 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 281 | 1.6-2.0 M MgCl2 and 0.1 M bicine pH 9.0 |
