6I9K
Crystal structure of Jumping Spider Rhodopsin-1 bound to 9-cis retinal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 50.955, 130.638, 77.309 |
| Unit cell angles | 90.00, 100.55, 90.00 |
Refinement procedure
| Resolution | 46.773 - 2.145 |
| R-factor | 0.2172 |
| Rwork | 0.215 |
| R-free | 0.25540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z73 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHENIX (1.13_2998) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.773 | 2.390 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.142 | 0.748 |
| Rmeas | 0.170 | |
| Rpim | 0.092 | |
| Number of reflections | 18992 | 938 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 90.6 | 51.7 |
| Redundancy | 6.1 | 3.5 |
| CC(1/2) | 0.995 | 0.537 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | PEG 400, Bis-Tris pH6.5 |
