6I9J

Human transforming growth factor beta2 in a tetragonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 41 21 2
Unit cell lengths	55.570, 55.570, 70.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.660 - 2.000
R-factor	0.218
Rwork	0.217
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2tgi
RMSD bond length	0.010
RMSD bond angle	1.180
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.600	2.120
High resolution limit [Å]	2.000	2.000
Rmerge	0.070	2.495
Rmeas	0.072	2.546
Number of reflections	7919
<I/σ(I)>	23.4	1.7
Completeness [%]	100.0	99.9
Redundancy	24.3	24.8
CC(1/2)	1.000	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	20% isopropanol, 0.2M calcium chloride, 0.1M sodium acetate pH 4.6