6I9G
Crystal structure of encapsulin from Mycolicibacterium hassiacum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 231.272, 242.149, 271.557 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.800 - 2.500 |
R-factor | 0.17422 |
Rwork | 0.172 |
R-free | 0.21048 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dkt |
RMSD bond length | 0.009 |
RMSD bond angle | 1.277 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.800 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.120 | 0.509 |
Rpim | 0.084 | 0.365 |
Number of reflections | 255440 | 12672 |
<I/σ(I)> | 7.1 | 2.4 |
Completeness [%] | 98.2 | 98.8 |
Redundancy | 3.6 | 3.5 |
CC(1/2) | 0.979 | 0.497 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 6.0 mg/ml encapsulin. 1.3 -1.6 M ammonium sulfate, 0.1 MES buffer pH 5.5 |