6I8X
As-p18, an extracellular fatty acid binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95369 |
| Spacegroup name | I 4 |
| Unit cell lengths | 102.254, 102.254, 103.392 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.410 - 2.300 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g74 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.060 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.104 | 0.679 |
| Rpim | 0.049 | 0.435 |
| Number of reflections | 23648 | |
| <I/σ(I)> | 9.8 | 1.8 |
| Completeness [%] | 93.2 | 77.1 |
| Redundancy | 3.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 40% ethylene glycol, 0.1 M acetate pH 4.5 |
