6I8R
Crystal structure of an ancient sequence-reconstructed Elongation factor Tu (node 170)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97242 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.225, 73.956, 115.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.420 - 2.000 |
R-factor | 0.1949 |
Rwork | 0.192 |
R-free | 0.24170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dg1 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.983 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.23) |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.420 | 45.420 | 2.050 |
High resolution limit [Å] | 2.000 | 8.930 | 2.000 |
Rmerge | 0.052 | 0.031 | 1.596 |
Rmeas | 0.055 | 0.033 | 1.686 |
Rpim | 0.018 | 0.012 | 0.534 |
Total number of observations | 325593 | ||
Number of reflections | 33564 | 447 | 2391 |
<I/σ(I)> | 17.5 | ||
Completeness [%] | 99.0 | 98 | 96.5 |
Redundancy | 9.7 | 8 | 9.6 |
CC(1/2) | 0.999 | 0.999 | 0.724 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 0.2 M Potassium Chloride, 20% w/v Polyethylene glycol 3350 |