6I8H

Structure of the plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody ENB15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 63 2 2
Unit cell lengths	145.887, 145.887, 152.770
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	72.940 - 3.682
R-factor	0.246
Rwork	0.242
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nfu
RMSD bond length	0.002
RMSD bond angle	0.608
Data reduction software	XDS (VERSION Jan 26, 2018)
Data scaling software	autoPROC (Version 1.0.5)
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.361	4.050
High resolution limit [Å]	3.682	3.682
Rmerge	0.111	1.349
Number of reflections	8246	411
<I/σ(I)>	9.8	1.5
Completeness [%]	93.0	59.5
Redundancy	6.3	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277.15	Reservoir composition: 12.5 % (v/v) (RS)-2-methyl-2,4-pentanediol, 12.5 % (w/v) PEG 1000, 12.5 % (w/v) PEG3350, 0.03 M magnesium chloride, 0.03 M calcium chloride, 0.0612 M MES, 0.0388 M imidazole, pH 6.5; protein stock solution: 4.1 mg/ml protein, 50 mM sodium chloride, 1 % (v/v) glycerole, 1 mM DTT, 50 mM HEPES, pH 8.0; drop composition: 150 nl protein stock solution plus 225 nl reservoir solution; cryo conditions: the crystals were flash frozen directly from the equilibrated crystallization drops.