6I8G

Structure of the plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody ENB73

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-28
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.967700
Spacegroup name	C 1 2 1
Unit cell lengths	177.504, 68.232, 105.273
Unit cell angles	90.00, 123.44, 90.00

Refinement procedure

Resolution	52.570 - 2.344
R-factor	0.195
Rwork	0.194
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nfu
RMSD bond length	0.002
RMSD bond angle	0.522
Data reduction software	XDS (VERSION Jan 26, 2018)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.773	2.565
High resolution limit [Å]	2.344	2.344
Rmerge	0.117	0.870
Number of reflections	31550	1577
<I/σ(I)>	8	1.5
Completeness [%]	71.1	15.1
Redundancy	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	Reservoir composition: 17.5 % (w/v) PEG3350, 0.2 M sodium citrate, 0.1 M Bis-Tris buffer, pH 8.5; Protein stock solution: 2.8 mg/ml protein in 50 mM sodium chloride, 1 % (v/v) glycerole, 1mM DTT, 50 mM HEPES, pH 8.0; drop composition: 1 mikroliter protein stock solution + 1 mikroliter reservoir solution; crystals were cryoprotected in 17.5 % (w/v) PEG3350, 20 % (v/v) ethylene glycol, 0.2 M sodium citrate, 0.1 M Bis-Tris buffer, pH 8.5.