6I7K
Crystal structure of monomeric FICD mutant L258D complexed with MgATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 186.842, 186.842, 76.844 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 93.420 - 2.540 |
| R-factor | 0.23356 |
| Rwork | 0.233 |
| R-free | 0.25206 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u0u |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.130 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.420 | 2.650 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.167 | 1.009 |
| Rmeas | 0.178 | 1.073 |
| Rpim | 0.060 | 0.364 |
| Number of reflections | 26617 | 3188 |
| <I/σ(I)> | 13 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.1 | 16.5 |
| CC(1/2) | 0.999 | 0.503 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.0 M Sodium Chloride; 10% Ethanol |
