6I7G
Crystal structure of dimeric wild type FICD complexed with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-15 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.671, 107.652, 132.597 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 83.580 - 2.700 |
R-factor | 0.28458 |
Rwork | 0.283 |
R-free | 0.31868 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u0u |
RMSD bond length | 0.002 |
RMSD bond angle | 1.143 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.580 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.163 | 0.717 |
Rmeas | 0.195 | 0.851 |
Rpim | 0.104 | 0.456 |
Number of reflections | 31293 | 4091 |
<I/σ(I)> | 6 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.4 | 6.5 |
CC(1/2) | 0.996 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Tris pH 7.5; 20% PEG 300; 5% PEG8K; 10% Glycerol |