6I6Y

Circular permutant of ribosomal protein S6, swap helix 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97996
Spacegroup name	P 41 21 2
Unit cell lengths	34.810, 34.810, 134.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.690 - 2.150
R-factor	0.2303
Rwork	0.228
R-free	0.26810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ris
RMSD bond length	0.017
RMSD bond angle	1.771
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	134.190	134.190	2.220
High resolution limit [Å]	2.150	8.880	2.150
Rmerge	0.123	0.077	1.364
Rmeas	0.129	0.082	1.421
Rpim	0.039	0.027	0.392
Total number of observations	57725
Number of reflections	5024	104	422
<I/σ(I)>	9.3
Completeness [%]	100.0	98.3	100
Redundancy	11.5	6.9	12.7
CC(1/2)	0.998	0.999	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.02 M Magnesium chloride hexahydrate 0.1 M HEPES 7.5 pH 22% w/v Poly (acrylic acid sodium salt) 5100