6I6Y
Circular permutant of ribosomal protein S6, swap helix 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97996 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 34.810, 34.810, 134.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.690 - 2.150 |
| R-factor | 0.2303 |
| Rwork | 0.228 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ris |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.771 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 134.190 | 134.190 | 2.220 |
| High resolution limit [Å] | 2.150 | 8.880 | 2.150 |
| Rmerge | 0.123 | 0.077 | 1.364 |
| Rmeas | 0.129 | 0.082 | 1.421 |
| Rpim | 0.039 | 0.027 | 0.392 |
| Total number of observations | 57725 | ||
| Number of reflections | 5024 | 104 | 422 |
| <I/σ(I)> | 9.3 | ||
| Completeness [%] | 100.0 | 98.3 | 100 |
| Redundancy | 11.5 | 6.9 | 12.7 |
| CC(1/2) | 0.998 | 0.999 | 0.789 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.02 M Magnesium chloride hexahydrate 0.1 M HEPES 7.5 pH 22% w/v Poly (acrylic acid sodium salt) 5100 |
