6I6W
Circular permutant of ribosomal protein S6, adding 6aa to C terminal of P68-69
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 34.950, 34.950, 134.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.820 - 1.490 |
| R-factor | 0.1749 |
| Rwork | 0.172 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ris |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.452 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 134.030 | 134.030 | 1.520 |
| High resolution limit [Å] | 1.490 | 8.170 | 1.490 |
| Rmerge | 0.088 | 0.073 | 1.649 |
| Rmeas | 0.090 | 0.077 | 1.690 |
| Rpim | 0.019 | 0.022 | 0.362 |
| Total number of observations | 316130 | ||
| Number of reflections | 14490 | 128 | 698 |
| <I/σ(I)> | 15.5 | ||
| Completeness [%] | 100.0 | 95.4 | 100 |
| Redundancy | 21.8 | 12.8 | 21.4 |
| CC(1/2) | 0.999 | 0.994 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.0 M Succinic acid 0.1 M HEPES pH 7.0 1% w/v PEG 2000 MME |
