6I6S
Circular permutant of ribosomal protein S6, adding 9aa to C terminal of P68-69, L75A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-31 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97999 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 48.730, 52.900, 133.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.840 - 1.460 |
R-factor | 0.1674 |
Rwork | 0.166 |
R-free | 0.19600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ris |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.2) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 133.060 | 133.060 | 1.480 |
High resolution limit [Å] | 1.460 | 7.990 | 1.460 |
Rmerge | 0.100 | 0.070 | 1.178 |
Rmeas | 0.104 | 0.074 | 1.225 |
Rpim | 0.029 | 0.024 | 0.334 |
Total number of observations | 387834 | ||
Number of reflections | 30436 | 226 | 1495 |
<I/σ(I)> | 11.6 | ||
Completeness [%] | 100.0 | 97.2 | 100 |
Redundancy | 12.7 | 8.6 | 13.3 |
CC(1/2) | 0.998 | 0.995 | 0.826 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Potassium sodium tartrate tetrahydrate 0.1 M Bis-Tris propane pH 7.5 20% w/v PEG 3350 |