6I6S

Circular permutant of ribosomal protein S6, adding 9aa to C terminal of P68-69, L75A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97999
Spacegroup name	C 2 2 21
Unit cell lengths	48.730, 52.900, 133.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.840 - 1.460
R-factor	0.1674
Rwork	0.166
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ris
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	133.060	133.060	1.480
High resolution limit [Å]	1.460	7.990	1.460
Rmerge	0.100	0.070	1.178
Rmeas	0.104	0.074	1.225
Rpim	0.029	0.024	0.334
Total number of observations	387834
Number of reflections	30436	226	1495
<I/σ(I)>	11.6
Completeness [%]	100.0	97.2	100
Redundancy	12.7	8.6	13.3
CC(1/2)	0.998	0.995	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Potassium sodium tartrate tetrahydrate 0.1 M Bis-Tris propane pH 7.5 20% w/v PEG 3350