6I6O
Circular permutant of ribosomal protein S6, swap helix 2, L75A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.424, 76.461, 127.519 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.580 - 1.900 |
| R-factor | 0.1943 |
| Rwork | 0.192 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ris |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.813 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.580 | 65.580 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.090 | 0.048 | 0.387 |
| Rmeas | 0.094 | 0.051 | 0.404 |
| Rpim | 0.027 | 0.016 | 0.114 |
| Total number of observations | 414291 | ||
| Number of reflections | 34312 | 380 | 2143 |
| <I/σ(I)> | 15.8 | ||
| Completeness [%] | 99.9 | 99.7 | 99.6 |
| Redundancy | 12.1 | 9.9 | 12.4 |
| CC(1/2) | 0.999 | 0.998 | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Sodium fluoride 20% w/v PEG 3350 |
