6I6O

Circular permutant of ribosomal protein S6, swap helix 2, L75A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	43.424, 76.461, 127.519
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.580 - 1.900
R-factor	0.1943
Rwork	0.192
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ris
RMSD bond length	0.019
RMSD bond angle	1.813
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.32)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.580	65.580	1.940
High resolution limit [Å]	1.900	9.110	1.900
Rmerge	0.090	0.048	0.387
Rmeas	0.094	0.051	0.404
Rpim	0.027	0.016	0.114
Total number of observations	414291
Number of reflections	34312	380	2143
<I/σ(I)>	15.8
Completeness [%]	99.9	99.7	99.6
Redundancy	12.1	9.9	12.4
CC(1/2)	0.999	0.998	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Sodium fluoride 20% w/v PEG 3350