6I6I
Circular permutant of ribosomal protein S6, adding 6aa to C terminal of P68-69, L75A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 48.651, 48.659, 132.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.250 - 1.500 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ris |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.892 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.250 | 44.250 | 1.520 |
| High resolution limit [Å] | 1.500 | 8.200 | 1.500 |
| Rmerge | 0.086 | 0.063 | 0.630 |
| Rmeas | 0.090 | 0.066 | 0.659 |
| Rpim | 0.026 | 0.021 | 0.193 |
| Total number of observations | 304038 | ||
| Number of reflections | 25756 | 177 | 1237 |
| <I/σ(I)> | 12.5 | ||
| Completeness [%] | 99.6 | 93.5 | 99.2 |
| Redundancy | 11.8 | 9.5 | 11.2 |
| CC(1/2) | 0.998 | 0.995 | 0.655 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Lithium sulphate 0.1 M Tris pH 8.5 40% v/v PEG 400 |
