6I6I

Circular permutant of ribosomal protein S6, adding 6aa to C terminal of P68-69, L75A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	C 2 2 21
Unit cell lengths	48.651, 48.659, 132.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.250 - 1.500
R-factor	0.192
Rwork	0.190
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ris
RMSD bond length	0.019
RMSD bond angle	1.892
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.32)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.250	44.250	1.520
High resolution limit [Å]	1.500	8.200	1.500
Rmerge	0.086	0.063	0.630
Rmeas	0.090	0.066	0.659
Rpim	0.026	0.021	0.193
Total number of observations	304038
Number of reflections	25756	177	1237
<I/σ(I)>	12.5
Completeness [%]	99.6	93.5	99.2
Redundancy	11.8	9.5	11.2
CC(1/2)	0.998	0.995	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Lithium sulphate 0.1 M Tris pH 8.5 40% v/v PEG 400