6I6E

Circular permutant of ribosomal protein S6, swap strand 1 , L10A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 41 21 2
Unit cell lengths	34.870, 34.870, 135.149
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.790 - 1.200
R-factor	0.1455
Rwork	0.145
R-free	0.16270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ris
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.27)
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.050	45.050	1.220
High resolution limit [Å]	1.200	6.570	1.200
Rmerge	0.060	0.047	0.654
Rmeas	0.061	0.049	0.668
Rpim	0.012	0.013	0.135
Total number of observations	658750
Number of reflections	27269	228	1296
<I/σ(I)>	23.5
Completeness [%]	100.0	96.2	100
Redundancy	24.2	15.9	24
CC(1/2)	1.000	0.998	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.02 M Magnesium chloride hexahydrate 0.1 M HEPES pH 7.5 22% w/v Poly (acrylic acid sodium salt) 5100