6I60
Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 173.220, 161.530, 124.380 |
Unit cell angles | 90.00, 132.14, 90.00 |
Refinement procedure
Resolution | 43.280 - 2.743 |
R-factor | 0.2279 |
Rwork | 0.226 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w5m |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.0) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.280 | 43.280 | 2.910 |
High resolution limit [Å] | 2.740 | 8.130 | 2.740 |
Rmerge | 0.180 | 0.051 | 0.937 |
Rmeas | 0.210 | 0.060 | 1.112 |
Number of reflections | 65285 | 2542 | 10109 |
<I/σ(I)> | 7.68 | 24.47 | 1.39 |
Completeness [%] | 97.9 | 95.6 | 94.2 |
Redundancy | 3.725 | 3.656 | 3.394 |
CC(1/2) | 0.985 | 0.996 | 0.503 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG 1500 |