6I60

Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	173.220, 161.530, 124.380
Unit cell angles	90.00, 132.14, 90.00

Refinement procedure

Resolution	43.280 - 2.743
R-factor	0.2279
Rwork	0.226
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w5m
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.0)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.280	43.280	2.910
High resolution limit [Å]	2.740	8.130	2.740
Rmerge	0.180	0.051	0.937
Rmeas	0.210	0.060	1.112
Number of reflections	65285	2542	10109
<I/σ(I)>	7.68	24.47	1.39
Completeness [%]	97.9	95.6	94.2
Redundancy	3.725	3.656	3.394
CC(1/2)	0.985	0.996	0.503

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% PEG 1500