6I5W

BlMnBP1 binding protein of an ABC transporter from Bifidobacterium animalis subsp. lactis ATCC27673 in complex with mannobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-17
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.9989
Spacegroup name	P 21 21 21
Unit cell lengths	53.520, 73.250, 103.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.551 - 1.900
R-factor	0.2134
Rwork	0.210
R-free	0.27310
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.551	2.000
High resolution limit [Å]	1.900	1.900
Number of reflections	32748
<I/σ(I)>	10.09	0.94
Completeness [%]	99.5
Redundancy	13.3
CC(1/2)	0.998	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	278	Mother liquor, 0.1 M MES pH 5.5, 0.05 M Zn-acetate and 18% PEG 8000. Protein,15 mg/ml in 10 mM MES pH 6.5 and 150 mM NaCl. Drops set up 1 to 1