6I59

Long wavelength native-SAD phasing of Sen1 helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-12
Detector	DECTRIS EIGER X 4M
Wavelength(s)	2.7
Spacegroup name	P 21 21 2
Unit cell lengths	91.830, 171.600, 69.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.551 - 2.950
R-factor	0.1714
Rwork	0.169
R-free	0.21270
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.658
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXCD
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.030
High resolution limit [Å]	2.950	2.950
Rmeas	0.032	0.428
Number of reflections	23557	1653
<I/σ(I)>	31.08	1.66
Completeness [%]	99.1	95.2
Redundancy	49.12	37.88
CC(1/2)	0.999	0.693

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	18% PEG 2K 0.1M MOPS 7.2