6I50

Structure of Eiger TNF from S. frugiperda

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	H 3
Unit cell lengths	55.222, 55.222, 142.096
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.365 - 1.690
R-factor	0.1565
Rwork	0.155
R-free	0.18200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u5y
RMSD bond length	0.004
RMSD bond angle	0.766
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.400	1.720
High resolution limit [Å]	1.690	1.690
Number of reflections	17657	757
<I/σ(I)>	10.1	1.2
Completeness [%]	97.5	81.2
Redundancy	8.6	3.5
CC(1/2)	0.997	0.401

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.09 M Sodium nitrate, 0.09 M Sodium phosphate dibasic, 0.09M Ammonium sulfate, 0.1 M Imidazole/MES, pH 6.5, 30% ethylene glycol/PEG8k