6I4Y
X-ray structure of the human mitochondrial PRELID3b-TRIAP1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 93.020, 133.000, 146.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.230 - 2.910 |
| R-factor | 0.21394 |
| Rwork | 0.210 |
| R-free | 0.29635 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xzv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.468 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.230 | 3.014 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Number of reflections | 20182 | |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 99.6 | |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | Tacsimate (60% v/v) pH 7 |
